Plasmid

Part:BBa_K5218006:Design

Designed by: Amy Fu, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-08-24)


pS1300-RtTAL-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1015
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 271
    Illegal BglII site found at 304
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1015
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No



Source

Team BGI-MammothEdu-South 2024 retrieved the RtTAL CDS from published article [1], and obtained the sequence through De novo DNA synthesis.

References